Get Genomics Protocols PDF

By Kaisa Silander, Janna Saarela (auth.), Mike Starkey, Ramnath Elaswarapu (eds.)

ISBN-10: 158829871X

ISBN-13: 9781588298713

ISBN-10: 1597451886

ISBN-13: 9781597451888

Since the 1st variation, released in 2001, genomics examine has taken nice strides. In Genomics Protocols, second Edition, a staff of specialist researchers percentage the most up-tp-date info in a box that has lately switched emphasis from gene identity to useful genomics and the characterization of genes and gene items. This quantity methods its complicated topic with a large point of view to provide its reader with a necessary review of genomics and its spinoff fields, with a spotlight on pivotal concerns similar to facts research. Following the hugely profitable tools in Molecular Biology™ sequence layout, the chapters offer easy-to-follow laboratory protocols, lists of the mandatory fabrics, and the wonderful Notes part, which bargains pointers on troubleshooting and keeping off identified pitfalls.

Expansive and present, Genomics Protocols, second Edition is the ideal replace for researchers operating during this critical, transforming into field.

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Extra resources for Genomics Protocols

Example text

Figure 4, lanes 5 and 6, show two WGA DNA samples in which the last inactivation step was not done. The sex-PCR test failed for both samples. Following the inactivation step for these two samples (lanes 2 and 3), the sex-PCR test was successful. References 1. Nelson DL, Ledbetter SA, Corbo L, Victoria MF, Ramirez-Solis R, Webster TD, Ledbetter DH, Caskey CT (1989) Alu polymerase chain reaction: A method for rapid isolation of humanspecific sequences from complex DNA sources. Proc Natl Acad Sci USA 86:6686–6690 2.

Am J Pathol 164:23–33 16. Ballantyne KN, van Oorschot RA, Mitchell RJ (2007) Comparison of two whole genome amplification methods for STR genotyping of LCN and degraded DNA samples. Forensic Sci Int 166:35–41 17. Short AD, Kennedy LJ, Forman O, Barnes A, Fretwell N, Wiggall R, Thomson W, and Ollier WE (2005) Canine DNA subjected to whole genome amplification is suitable for a wide range of molecular applications. J Hered 96:829–835 18. Gorrochotegui-Escalante N, Black WCT (2003) Amplifying whole insect genomes with multiple displacement amplification.

Li Q, Deka C, Glassner B, Arnold K, Li-Sucholeiki X, Tomita-Mitchell A, Thilly W, Karger B (2005) Design of an automated multicapillary instrument with fraction collection for DNA mutation discovery by constant denaturant capillary electrophoresis (CDCE). J Separation Science 28:1375–1389 40. Bjørheim J, Gaudernack G, Giercksky KE, Ekstrøm PO (2003) Direct identification of all oncogenic mutants in KRAS exon 1 by cycling temperature capillary electrophoresis. Electrophoresis 24:63–69 41. Fodde R, Losekoot M (1994) Mutation detection by denaturing gradient gel electrophoresis (DGGE).

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Genomics Protocols by Kaisa Silander, Janna Saarela (auth.), Mike Starkey, Ramnath Elaswarapu (eds.)


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