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Extra info for Current Protocols in Protein Science: 001
Prepare the apparatus according to the manufacturer’s instructions using the ultrafiltration cell from Amicon either an Amicon Ultracel YM 30 or Ultracel PLTK membrane. Slowly stir the contents during the concentration process, which is carried out at 4°C. Continue until the required volume remains in the cell. Earlier work used Amicon Diaflo XM-50 membranes for this procedure, but these are no longer readily available. The Ultracel Amicon YM 30 or Ultracel PLTK membranes are appropriate substitutes.
Protein Purification, Principles and Practice, 3rd ed. Springer-Verlag, New York and Heidelberg. General principles of all the main techniques used in purifying proteins. A useful laboratory handbook; does not include recipes or procedures for specific proteins. Contributed by R. K. 4 Because of the wide variety of procedures that may be used to purify any given enzyme, it is not possible to provide a single protocol that includes them all. 3:GDH)—have been selected as examples because they present two very different approaches to the purification of the enzyme.
4 Current Protocols in Protein Science Protein Purification Flow Charts Protein purification flow charts are presented to give a broad outline of the methods used for different types of proteins. They cannot give any detail, as the process appropriate for each protein will have its own variations at each stage. In most cases, the first stage is to obtain a solution containing the desired protein, after which it can be dealt with by the many separation techniques described in the following chapters.
Current Protocols in Protein Science: 001 by COLIGAN