David W. Murhammer (auth.), David W. Murhammer (eds.)'s Baculovirus and Insect Cell Expression Protocols PDF

By David W. Murhammer (auth.), David W. Murhammer (eds.)

ISBN-10: 1588295370

ISBN-13: 9781588295378

Baculovirus Expression Protocols is a close consultant for utilizing the baculovirus expression vector approach (BEVS) and/or insect cells to supply recombinant proteins. approaches for the improvement and construction of baculovirus pesticides at either laboratory and massive scale are defined within the type of step by step structure that the tools in Molecular Biology™ sequence helped standardize. This e-book is a one-stop resource for info on baculoviruses, and comprises sections on utilizing transformed baculoviruses to precise genes in mammalian cells, utilizing Drosophila telephone strains, separating new telephone traces, and constructing serum-free medium. particular purposes, together with utilizing baculovirus and bug cellphone approach to review apoptosis, also are mentioned. This up-to-date and multiplied variation of Baculovirus Expression Protocols offers an ideal lab handbook for biochemists, molecular biologists, biochemical engineers, and others for utilizing the BEVS or insect mobilephone strains to provide recombinant proteins.

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Baculovirus with a deletion of vlf-1. Virology 326, 191–201. 122 Guarino, L. , and Dong, W. (1998) A virus-encoded RNA poly122. merase purified from baculovirus-infected cells. J. Virol. 72, 7985–7991. 123 123. Fuchs, L. , Woods, M. , and Weaver, R. F. (1983) Viral transcription during Autographa californica nuclear polyhedrosis virus infection: a novel RNA polymerase induced in infected Spodoptera frugiperda cells. J. Virol. 48, 641–646. 124 Guarino, L. , and Dong, W. (1998) Guanylyltransferase activity of the 124.

This viral polymerase is α-amanitin-resistant and unable to transcribe from early gene promoters (123). The products of four lef genes, lef-8, lef-4, lef-9, and p47, have been identified as components of the AcMNPV RNA polymerase (122). The LEF-4 has guanylytransferase activity (124) and RNA 5' triphosphatase and nucleoside triphosphatase activity (125). Genes encoding each of the RNA polymerase subunits have been shown to be necessary for late and very late gene expression in transient expression assays (113,126,127).

K. (2000) The inhibitors of apoptosis of Epiphyas postvittana nucleopolyhedrovirus. J. Gen. Virol. 81, 2803–2811. , Park, Y. , Rich, R. , Myszka, D. , and Wu, H. (2001) Structural basis of caspase inhibition by XIAP: differential roles of the linker versus the BIR domain. Cell 104, 781–790. Seshagiri, S. and Miller, L. K. (1997) Baculovirus inhibitors of apoptosis (IAPs) block activation of Sf-caspase-1. Proc. Natl. Acad. Sci. USA 94, 13,606–13,611. , Voeten, J. T. , Goldbach, R. , and Vlak, J.

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Baculovirus and Insect Cell Expression Protocols by David W. Murhammer (auth.), David W. Murhammer (eds.)

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